Hotspot

rrn5-rrn4.5

IRa · 134810-135026 · 217 bp

Back to Cyrtanthus

Pi 0.0415

MarkerSeek score 74.50

Primer available yes

Label rrn5-rrn4.5

Feature Metadata

label_name: rrn5-rrn4.5
feature_type: igs
region: IRa
length_bp: 217
variable_sites: 9
pi: 0.0415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rrn4.5_p1	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	334 bp	1.0	81.5
rrn5-rrn4.5_p2	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	332 bp	1.0	81.5
rrn5-rrn4.5_p3	AGTATCGTCACCGCAGTAGA	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	332 bp	1.0	81.5
rrn5-rrn4.5_p4	TATCGTCACCGCAGTAGAGT	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	330 bp	1.0	81.5
rrn5-rrn4.5_p5	TCCCCTACAGTATCGTCACC	CAGCTGAGGCATCCTAACAG	55.0	55.0	57.9	58.0	342 bp	1.0	81.2