Hotspot

rps15-ycf1

SSC · 127553-127917 · 365 bp

Back to Cyrtanthus

Pi 0.022

MarkerSeek score 55.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 365
variable_sites: 8
pi: 0.022
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCGAATAGCTGAGATGAATGCA	GATACCACTAACGGCAGTCG	40.9	55.0	57.7	58.2	467-492 bp	1.0	61.6
rps15-ycf1_p2	TCGAATAGCTGAGATGAATGCA	CGGCAGTCGTTTCAGTATGT	40.9	50.0	57.7	58.3	456-481 bp	1.0	60.6
rps15-ycf1_p3	GTCGAATAGCTGAGATGAATGC	GATACCACTAACGGCAGTCG	45.5	55.0	57.4	58.2	468-493 bp	1.0	59.0
rps15-ycf1_p4	GTCGAATAGCTGAGATGAATGC	CGGCAGTCGTTTCAGTATGT	45.5	50.0	57.4	58.3	457-482 bp	1.0	58.1
rps15-ycf1_p5	CGAATAGCTGAGATGAATGCA	GATACCACTAACGGCAGTCG	42.9	55.0	56.2	58.2	466-491 bp	1.0	57.9