Hotspot

ndhF-rpl32

SSC · 116115-117063 · 949 bp

Back to Cyrtanthus

Pi 0.0107

MarkerSeek score 66.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 949
variable_sites: 10
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGCTGTCGGAATAAACAGAAGT	AGAAAAAGCTTTTATGGCAGC	40.9	38.1	58.0	55.6	1134-1144 bp	1.0	41.6
ndhF-rpl32_p2	TGTTGCTGTCGGAATAAACAG	AGAAAAAGCTTTTATGGCAGC	42.9	38.1	56.8	55.6	1137-1147 bp	1.0	41.6
ndhF-rpl32_p3	TGCTGTCGGAATAAACAGAAGT	AAGAAAAAGCTTTTATGGCAGC	40.9	36.4	58.0	56.4	1135-1145 bp	1.0	41.6
ndhF-rpl32_p4	TGTTGCTGTCGGAATAAACAGA	AGAAAAAGCTTTTATGGCAGC	40.9	38.1	58.3	55.6	1137-1147 bp	1.0	41.6
ndhF-rpl32_p5	TGCTGTCGGAATAAACAGAAGT	TGGCAGCTAAATATCCCTTT	40.9	40.0	58.0	54.4	1120-1130 bp	1.0	41.6