Hotspot

trnR-ACG-trnN-GUU

IRb · 111948-112504 · 557 bp

Back to Cyrtanthus

Pi 0.00721

MarkerSeek score 62.30

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 557
variable_sites: 4
pi: 0.00721
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	696-698 bp	1.0	78.9
trnR-ACG-trnN-GUU_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	733-735 bp	1.0	78.3
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	647-649 bp	1.0	77.8
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	CTGGTCGTAGGTTCGAATCC	55.0	55.0	57.8	57.8	648-650 bp	1.0	77.4
trnR-ACG-trnN-GUU_p5	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.7	58.2	684-686 bp	1.0	77.2