Hotspot

rps12-trnV-GAC

IRb · 102254-104072 · 1819 bp

Back to Cyrtanthus

Pi 0.00661
MarkerSeek score 55.30
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1819
variable_sites
12
pi
0.00661
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1915 bp 1.0 77.3
rps12-trnV-GAC_p2 TGGCAACTTTACGTAAGGCA AACTGATGACTTCCACCACG 45.0 50.0 57.7 57.8 1919 bp 1.0 77.2
rps12-trnV-GAC_p3 TGGCAACTTTACGTAAGGCA ATGACTTCCACCACGTCAAG 45.0 50.0 57.7 57.8 1914 bp 1.0 77.2
rps12-trnV-GAC_p4 TGGCAACTTTACGTAAGGCA CTGATGACTTCCACCACGTC 45.0 55.0 57.7 58.3 1917 bp 1.0 76.3
rps12-trnV-GAC_p5 TGGCAACTTTACGTAAGGCA GTCAAGGTGACACTCTACCG 45.0 55.0 57.7 57.7 1900 bp 1.0 75.8