Hotspot

petD-rpoA

LSC · 81084-81235 · 152 bp

Back to Cyrtanthus

Pi 0.0199

MarkerSeek score 64.50

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 3
pi: 0.0199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TGGGGCAACGTTACCTATTG	ACTCTACAGAAGCATTTCGCA	50.0	42.9	57.9	57.7	294-340 bp	1.0	69.5
petD-rpoA_p2	AGGTATTGGGGCAACGTTAC	ACTCTACAGAAGCATTTCGCA	50.0	42.9	57.9	57.7	300-346 bp	1.0	69.5
petD-rpoA_p3	GTATTGGGGCAACGTTACCT	ACTCTACAGAAGCATTTCGCA	50.0	42.9	57.9	57.7	298-344 bp	1.0	69.5
petD-rpoA_p4	GTTAGGTATTGGGGCAACGT	ACTCTACAGAAGCATTTCGCA	50.0	42.9	57.9	57.7	303-349 bp	1.0	69.5
petD-rpoA_p5	CCGCGAAATACCACGATGTA	ACTCTACAGAAGCATTTCGCA	50.0	42.9	58.2	57.7	235-281 bp	1.0	69.4