Hotspot

petG-trnW-CCA

LSC · 69502-69616 · 115 bp

Back to Cyrtanthus

Pi 0.0566

MarkerSeek score 85.60

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 115
variable_sites: 6
pi: 0.0566
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAATACAGACGCGGTGATC	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	184-188 bp	1.0	85.1
petG-trnW-CCA_p2	CAATACAGACGCGGTGATCA	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	183-187 bp	1.0	85.1
petG-trnW-CCA_p3	ACAATACAGACGCGGTGATC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	220-224 bp	1.0	84.7
petG-trnW-CCA_p4	CAATACAGACGCGGTGATCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	219-223 bp	1.0	84.7
petG-trnW-CCA_p5	CGGTGATCAGTTGGACCTTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	208-212 bp	1.0	84.1