Hotspot

rbcL-accD

LSC · 59925-60704 · 780 bp

Back to Cyrtanthus

Pi 0.0144

MarkerSeek score 66.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 780
variable_sites: 11
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCAAATGGAGCCCTGAACTA	CTTCCACCGGTATGTCCAAG	50.0	55.0	57.9	58.0	971-985 bp	1.0	80.8
rbcL-accD_p2	GCAAATGGAGCCCTGAACTA	ACATCGAATTGAACCACCGT	50.0	45.0	57.9	57.8	889-903 bp	1.0	79.8
rbcL-accD_p3	CGCTGCTTGTGAAGTATGGA	CTTCCACCGGTATGTCCAAG	50.0	55.0	58.3	58.0	949-963 bp	1.0	79.7
rbcL-accD_p4	CGCTGCTTGTGAAGTATGGA	ACATCGAATTGAACCACCGT	50.0	45.0	58.3	57.8	867-881 bp	1.0	78.7
rbcL-accD_p5	AAATGGAGCCCTGAACTAGC	CTTCCACCGGTATGTCCAAG	50.0	55.0	57.6	58.0	969-983 bp	1.0	78.4