Hotspot

trnK-UUU-rps16

LSC · 4050-4664 · 615 bp

Back to Cyrtanthus

Pi 0.00334
MarkerSeek score 61.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
615
variable_sites
2
pi
0.00334
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCTGGACGAATTCCTTGC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.9 58.2 770-777 bp 1.0 79.5
trnK-UUU-rps16_p2 GTCTGGACGAATTCCTTGCT GGTGCTCAACCTACAGGAAC 50.0 55.0 57.9 58.2 769-776 bp 1.0 79.5
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 714-721 bp 1.0 79.1
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 713-720 bp 1.0 79.1
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.5 58.2 700-707 bp 1.0 76.8