Hotspot

psaC-ndhE

SSC · 156836-159818 · 2983 bp

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Pi 0.21
MarkerSeek score 71.40
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
2983
variable_sites
1202
pi
0.21
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.66
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 AAGGGCCAAGATCCTTTGAT TGGAATCCAACTTCTCTTCTTCT 45.0 39.1 56.8 57.2 847-2692 bp 0.555556 34.2
psaC-ndhE_p2 AGGGCCAAGATCCTTTGATT TGGAATCCAACTTCTCTTCTTCT 45.0 39.1 56.8 57.2 846-2691 bp 0.555556 34.2
psaC-ndhE_p3 AAGGGCCAAGATCCTTTGATT TGGAATCCAACTTCTCTTCTTCT 42.9 39.1 57.5 57.2 847-2692 bp 0.555556 34.2
psaC-ndhE_p4 AGGGCCAAGATCCTTTGATTT TGGAATCCAACTTCTCTTCTTCT 42.9 39.1 57.5 57.2 846-2691 bp 0.555556 34.2
psaC-ndhE_p5 AGGGCCAAGATCCTTTGATT TGGAATCCAACTTCTCTTCTTCTT 45.0 37.5 56.8 57.8 846-2691 bp 0.555556 34.2