Hotspot

trnL-UAG-ccsA

SSC · 152441-153673 · 1233 bp

Back to Cypripedium

Pi 0.234
MarkerSeek score 78.90
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
1233
variable_sites
683
pi
0.234
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.96
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA TCCGCATAAAAGAGCTGCAT 50.0 45.0 57.9 58.0 1166-2333 bp 0.555556 72.9
trnL-UAG-ccsA_p2 CATGAAGTGAGAAGCGTGGA ATCCGCATAAAAGAGCTGCA 50.0 45.0 57.9 58.0 2031-2629 bp 0.444444 68.8
trnL-UAG-ccsA_p3 CATGAAGTGAGAAGCGTGGA TCCGCATAAAAGAGCTGCAT 50.0 45.0 57.9 58.0 2030-2628 bp 0.444444 68.8
trnL-UAG-ccsA_p4 TCATGAAGTGAGAAGCGTGG ATCCGCATAAAAGAGCTGCA 50.0 45.0 57.9 58.0 2032-2630 bp 0.444444 68.8
trnL-UAG-ccsA_p5 TCATGAAGTGAGAAGCGTGG TCCGCATAAAAGAGCTGCAT 50.0 45.0 57.9 58.0 2031-2629 bp 0.444444 68.8