Hotspot

trnL-CAA-ndhB

IRb · 130636-131232 · 597 bp

Back to Cypripedium

Pi 0.0472

MarkerSeek score 86.90

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 597
variable_sites: 94
pi: 0.0472
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	617-1027 bp	1.0	91.5
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	615-1025 bp	1.0	91.5
trnL-CAA-ndhB_p3	GTCTCGCGTGTCTACCATTT	CCCGATTCTTGCAATTGCTC	50.0	50.0	58.0	57.8	626-1036 bp	1.0	90.3
trnL-CAA-ndhB_p4	CTCGCGTGTCTACCATTTCA	CCCGATTCTTGCAATTGCTC	50.0	50.0	58.0	57.8	624-1034 bp	1.0	90.3
trnL-CAA-ndhB_p5	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	612-1022 bp	1.0	90.0