Hotspot

rps8-rpl14

LSC · 111123-114138 · 3016 bp

Back to Cypripedium

Pi 0.212
MarkerSeek score 73.20
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
3016
variable_sites
1354
pi
0.212
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.72
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 TGCATGAGACACGATCTACT TCAGACTAGTAGGTGAAAATAGGA 45.0 37.5 56.1 56.1 785-2874 bp 0.444444 29.8
rps8-rpl14_p2 TGCATGAGACACGATCTACT CAGACTAGTAGGTGAAAATAGGA 45.0 39.1 56.1 54.7 784-2873 bp 0.444444 29.8
rps8-rpl14_p3 TGCATGAGACACGATCTACT TCAGACTAGTAGGTGAAAATAGG 45.0 39.1 56.1 54.7 785-2874 bp 0.444444 29.8
rps8-rpl14_p4 ATGCATGAGACACGATCTACT TCAGACTAGTAGGTGAAAATAGGA 42.9 37.5 56.6 56.1 786-2875 bp 0.444444 29.8
rps8-rpl14_p5 TGCATGAGACACGATCTACT TCAGACTAGTAGGTGAAAATAGGAT 45.0 36.0 56.1 56.5 785-2874 bp 0.444444 29.8