Hotspot

clpP1-psbB

LSC · 98234-101340 · 3107 bp

Back to Cypripedium

Pi 0.198

MarkerSeek score 77.20

Primer available yes

Label clpP1-psbB

Feature Metadata

label_name: clpP1-psbB
feature_type: igs
region: LSC
length_bp: 3107
variable_sites: 1386
pi: 0.198
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1-psbB_p1	GGAAACGTAACAAAACAATGGC	TGGAACAATCGTCCGAATCC	40.9	50.0	57.3	58.0	1936-2750 bp	0.555556	52.5
clpP1-psbB_p2	GGAAACGTAACAAAACAATGGC	GAACAATCGTCCGAATCCGA	40.9	50.0	57.3	58.1	1934-2748 bp	0.555556	51.9
clpP1-psbB_p3	GGAAACGTAACAAAACAATGGC	ACAATCGTCCGAATCCGATC	40.9	50.0	57.3	57.9	1932-2746 bp	0.555556	51.5
clpP1-psbB_p4	GGAAACGTAACAAAACAATGGC	TGAGCTATGAGCCTTCCAGT	40.9	50.0	57.3	58.1	1867-2681 bp	0.444444	47.1
clpP1-psbB_p5	GGAAACGTAACAAAACAATGGC	AGCTATGAGCCTTCCAGTCT	40.9	50.0	57.3	57.8	1865-2679 bp	0.444444	46.8