Hotspot

petA-psbJ

LSC · 84772-86404 · 1633 bp

Back to Cypripedium

Pi 0.132

MarkerSeek score 85.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1633
variable_sites: 566
pi: 0.132
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAAAAGGTTCAGTTGTACGA	TATGGCTCCTATTCCGGGTT	38.1	50.0	55.4	57.9	1440-2252 bp	0.666667	49.1
petA-psbJ_p2	TGAAAAGGTTCAGTTGTACGA	TTATGGCTCCTATTCCGGGT	38.1	50.0	55.4	57.9	1441-2253 bp	0.666667	49.1
petA-psbJ_p3	ACAGTTTGAAAAGGTTCAGTTGT	TATGGCTCCTATTCCGGGTT	34.8	50.0	57.3	57.9	1446-2258 bp	0.666667	48.0
petA-psbJ_p4	ACAGTTTGAAAAGGTTCAGTTGT	TTATGGCTCCTATTCCGGGT	34.8	50.0	57.3	57.9	1447-2259 bp	0.666667	48.0
petA-psbJ_p5	AGGTTCAGTTGTACGAAATGA	TATGGCTCCTATTCCGGGTT	38.1	50.0	55.2	57.9	1435-2247 bp	0.666667	47.3