Hotspot

rbcL-accD

LSC · 77067-77980 · 914 bp

Back to Cypripedium

Pi 0.0456

MarkerSeek score 83.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 914
variable_sites: 114
pi: 0.0456
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTCGACCCAGTGGATAAGCT	TCGAATTGAACCAACATTTTTCCA	50.0	33.3	58.4	58.1	834-2034 bp	1.0	55.6
rbcL-accD_p2	TCGACCCAGTGGATAAGCTA	TCGAATTGAACCAACATTTTTCCA	50.0	33.3	57.6	58.1	833-2033 bp	1.0	55.5
rbcL-accD_p3	TTCGACCCAGTGGATAAGCT	CGAATTGAACCAACATTTTTCCA	50.0	34.8	58.4	56.8	833-2033 bp	1.0	54.5
rbcL-accD_p4	TCGACCCAGTGGATAAGCTA	CGAATTGAACCAACATTTTTCCA	50.0	34.8	57.6	56.8	832-2032 bp	1.0	54.4
rbcL-accD_p5	CGACCCAGTGGATAAGCTAG	TCGAATTGAACCAACATTTTTCCA	55.0	33.3	57.2	58.1	832-2032 bp	1.0	52.7