Hotspot

petN-psbM

LSC · 37546-39657 · 2112 bp

Back to Cypripedium

Pi 0.203

MarkerSeek score 78.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 2112
variable_sites: 1006
pi: 0.203
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCCTATTTTGACTCTGCGCC	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.0	57.9	1698-2982 bp	0.888889	86.7
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.2	57.9	1592-2881 bp	0.888889	85.7
petN-psbM_p3	TTGACTCTGCGCCATTGATT	CGTTTATTGCTACTGCGCTG	45.0	50.0	58.2	57.9	1691-2975 bp	0.888889	85.5
petN-psbM_p4	TTTGACTCTGCGCCATTGAT	CGTTTATTGCTACTGCGCTG	45.0	50.0	58.2	57.9	1692-2976 bp	0.888889	85.5
petN-psbM_p5	TCCTATTTTGACTCTGCGCC	AACTTTGTTTGTGCATCCGC	50.0	45.0	58.0	58.1	2832-2954 bp	0.333333	63.1