Hotspot

rrn23-trnA-UGC

IRa · 138920-139091 · 172 bp

Back to Cyphostemma

Pi 0.0233
MarkerSeek score 65.90
Primer available yes
Label rrn23-trnA-UGC

Feature Metadata

label_name
rrn23-trnA-UGC
feature_type
igs
region
IRa
length_bp
172
variable_sites
4
pi
0.0233
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn23-trnA-UGC_p1 ATTCGGGAATCTCCGGATCT GGTTCGAGTCCGCTTATCTC 50.0 55.0 58.0 57.9 303 bp 1.0 79.9
rrn23-trnA-UGC_p2 ATTCGGGAATCTCCGGATCT GTTCGAGTCCGCTTATCTCC 50.0 55.0 58.0 57.9 302 bp 1.0 79.9
rrn23-trnA-UGC_p3 GTCTCTGGGTGCCTAGGTAT GGTTCGAGTCCGCTTATCTC 55.0 55.0 57.9 57.9 231 bp 1.0 79.7
rrn23-trnA-UGC_p4 GTCTCTGGGTGCCTAGGTAT GTTCGAGTCCGCTTATCTCC 55.0 55.0 57.9 57.9 230 bp 1.0 79.7
rrn23-trnA-UGC_p5 ATTCGGGAATCTCCGGATCT TTCGAGTCCGCTTATCTCCA 50.0 50.0 58.0 58.2 301 bp 1.0 79.0