Hotspot

rpl16-rps3

LSC · 85354-86654 · 1301 bp

Back to Cyphostemma

Pi 0.0162

MarkerSeek score 56.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1301
variable_sites: 21
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GCCCCGCTTCTATTTGTCTA	TGGATATTTGTAGACGAGGA	50.0	40.0	57.4	52.9	1428-1429 bp	1.0	41.5
rpl16-rps3_p2	TGTGATCCAAGCAGGTTCAA	TGGATATTTGTAGACGAGGA	45.0	40.0	57.3	52.9	1406-1407 bp	1.0	41.5
rpl16-rps3_p3	GCCCCGCTTCTATTTGTCTA	TGGATATTTGTAGACGAGGAA	50.0	38.1	57.4	53.8	1428-1429 bp	1.0	41.5
rpl16-rps3_p4	GCCCCGCTTCTATTTGTCTA	TTGGATATTTGTAGACGAGGA	50.0	38.1	57.4	53.8	1429-1430 bp	1.0	41.5
rpl16-rps3_p5	TGTGATCCAAGCAGGTTCAA	TGGATATTTGTAGACGAGGAA	45.0	38.1	57.3	53.8	1406-1407 bp	1.0	41.5