Hotspot

psbC-trnS-UGA

LSC · 38100-38365 · 266 bp

Back to Cyphostemma

Pi 0.0618

MarkerSeek score 85.10

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 266
variable_sites: 16
pi: 0.0618
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCAGCAGGATTTGAAAAGGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	57.6	58.1	405-412 bp	1.0	81.0
psbC-trnS-UGA_p2	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	409-416 bp	1.0	81.0
psbC-trnS-UGA_p3	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	408-415 bp	1.0	80.5
psbC-trnS-UGA_p4	GCAGCAGGATTTGAAAAGGG	GATGGCCGAGTGGTTGATAG	50.0	55.0	57.6	58.1	416-423 bp	1.0	80.4
psbC-trnS-UGA_p5	AGCTGCAGCAGGATTTGAAA	GATGGCCGAGTGGTTGATAG	45.0	55.0	58.4	58.1	420-427 bp	1.0	80.3