Hotspot

trnR-UCU-atpA

LSC · 11439-11626 · 188 bp

Back to Cyphostemma

Pi 0.0495

MarkerSeek score 72.10

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 9
pi: 0.0495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CTACCAAGACGTTCACCGAG	47.6	55.0	57.3	58.0	290-296 bp	1.0	70.4
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TTCACCGAGCAAGCAGAAAT	47.6	45.0	57.3	58.1	279-285 bp	1.0	69.9
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	TCTACCAAGACGTTCACCGA	47.6	50.0	57.3	58.4	291-297 bp	1.0	67.4
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	TTCTACCAAGACGTTCACCG	47.6	50.0	57.3	57.6	292-298 bp	1.0	67.1
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	CCGAGCAAGCAGAAATCCTT	47.6	50.0	57.3	58.5	275-281 bp	1.0	66.4