Hotspot

trnS-GCU-trnR-UCU

LSC · 9497-11366 · 1870 bp

Back to Cyphostemma

Pi 0.0129

MarkerSeek score 52.60

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1870
variable_sites: 24
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	GCTTTAGTCCACTCAGCCAT	GTGTCCTATCCATTAGACAATG	50.0	40.9	57.6	54.1	1923-1953 bp	1.0	41.2
trnS-GCU-trnR-UCU_p2	AGTCCACTCAGCCATCTCTC	GTGTCCTATCCATTAGACAATG	55.0	40.9	58.5	54.1	1918-1948 bp	1.0	41.2
trnS-GCU-trnR-UCU_p3	ATTAGCAATCCGCCGCTTTA	GTGTCCTATCCATTAGACAATG	45.0	40.9	58.0	54.1	1937-1967 bp	1.0	41.1
trnS-GCU-trnR-UCU_p4	ATTAGCAATCCGCCGCTTTA	TGTCCTATCCATTAGACAATG	45.0	38.1	58.0	52.4	1936-1966 bp	1.0	41.1
trnS-GCU-trnR-UCU_p5	ATTAGCAATCCGCCGCTTTA	GTGTCCTATCCATTAGACAAT	45.0	38.1	58.0	52.4	1937-1967 bp	1.0	41.1