Hotspot

trnN-GUU-trnR-ACG

IRa · 156016-158234 · 2219 bp

Back to Cyperus

Pi 0.128

MarkerSeek score 73.70

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 2219
variable_sites: 597
pi: 0.128
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	TTCCTTTTTGAGGGTGCTCC	55.0	50.0	58.0	58.0	2406-2538 bp	0.75	74.4
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	TAACTCGACGCCAGAATGTG	55.0	50.0	58.0	58.0	2703-2835 bp	0.75	74.3
trnN-GUU-trnR-ACG_p3	AATGAGACATGGAAGGGGGA	TTCCTTTTTGAGGGTGCTCC	50.0	50.0	58.0	58.0	2521-2653 bp	0.75	74.3
trnN-GUU-trnR-ACG_p4	AATGAGACATGGAAGGGGGA	TAACTCGACGCCAGAATGTG	50.0	50.0	58.0	58.0	2818-2950 bp	0.75	74.2
trnN-GUU-trnR-ACG_p5	CCGTTGTTCCTCAGTAGCTC	CGAAATCACATGGCTGAGGA	55.0	50.0	58.0	58.0	2430-2562 bp	0.75	74.2