Hotspot

rps15-trnN-GUU

IRa · 152888-155943 · 3056 bp

Back to Cyperus

Pi 0.141
MarkerSeek score 76.40
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
IRa
length_bp
3056
variable_sites
898
pi
0.141
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 ACATTTCGTCCCCAAGAACC CGGGTTCAGGGAGGTAAATG 50.0 55.0 58.1 58.0 1076-1093 bp 1.0 90.9
rps15-trnN-GUU_p2 ACATTTCGTCCCCAAGAACC GATGGGGAACTCGCTTTAGG 50.0 55.0 58.1 58.0 1013-1030 bp 1.0 90.9
rps15-trnN-GUU_p3 ACATTTCGTCCCCAAGAACC GTTCAGGGAGGTAAATGCGT 50.0 50.0 58.1 57.9 1073-1090 bp 1.0 90.4
rps15-trnN-GUU_p4 ACATTTCGTCCCCAAGAACC GCTTATATGCCATGGGAGGG 50.0 55.0 58.1 57.9 977-994 bp 1.0 90.2
rps15-trnN-GUU_p5 CATTTCGTCCCCAAGAACCT CGGGTTCAGGGAGGTAAATG 50.0 55.0 57.8 58.0 1075-1092 bp 1.0 90.1