Hotspot

rpl32-ndhF

SSC · 143780-145083 · 1304 bp

Back to Cyperus

Pi 0.0234
MarkerSeek score 65.10
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
1304
variable_sites
80
pi
0.0234
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 GCTAAAGAAGAGGCTTTTACTGC CACTTTGATGGAAGCGGACT 43.5 50.0 58.0 58.2 1930-2577 bp 0.875 54.6
rpl32-ndhF_p2 AAGAAGAGGCTTTTACTGCA CACTTTGATGGAAGCGGACT 40.0 50.0 54.9 58.2 1926-2573 bp 0.875 53.8
rpl32-ndhF_p3 AGAAGAGGCTTTTACTGCAA CACTTTGATGGAAGCGGACT 40.0 50.0 54.9 58.2 1925-2572 bp 0.875 53.8
rpl32-ndhF_p4 GCTAAAGAAGAGGCTTTTACTGC TCACTTTGATGGAAGCGGAC 43.5 50.0 58.0 58.2 1931-1940 bp 0.75 48.0
rpl32-ndhF_p5 AGAAGAGGCTTTTACTGCAA TCACTTTGATGGAAGCGGAC 40.0 50.0 54.9 58.2 1926-1935 bp 0.75 47.2