Hotspot

trnL-UAG-rpl32

SSC · 142807-143587 · 781 bp

Back to Cyperus

Pi 0.0244

MarkerSeek score 65.70

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: SSC
length_bp: 781
variable_sites: 54
pi: 0.0244
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	ACACTAGGCACTGCTTCCTA	TACTGGGTCCGGGGTTTTAT	50.0	50.0	58.1	58.0	1318-1970 bp	0.875	82.8
trnL-UAG-rpl32_p2	TACACTAGGCACTGCTTCCT	TACTGGGTCCGGGGTTTTAT	50.0	50.0	58.1	58.0	1319-1971 bp	0.875	82.7
trnL-UAG-rpl32_p3	CCTGATGTCGCAAAAGCTTG	TACTGGGTCCGGGGTTTTAT	50.0	50.0	58.0	58.0	1759-2411 bp	0.875	82.4
trnL-UAG-rpl32_p4	AGACCCGCATAAAAGAGCTG	TACTGGGTCCGGGGTTTTAT	50.0	50.0	58.0	58.0	1880-2532 bp	0.875	82.3
trnL-UAG-rpl32_p5	CCTGATGTCGCAAAAGCTTG	TCGAATCCTTCTACCTCCCC	50.0	55.0	58.0	57.9	1890-2542 bp	0.875	82.0