Hotspot

trnT-UGU-ccsA

SSC · 140401-141692 · 1292 bp

Back to Cyperus

Pi 0.0219
MarkerSeek score 61.70
Primer available yes
Label trnT-UGU-ccsA

Feature Metadata

label_name
trnT-UGU-ccsA
feature_type
igs
region
SSC
length_bp
1292
variable_sites
75
pi
0.0219
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-ccsA_p1 TCGGATTCTGGATCACGAGA CAGCTCTTTTATGCGGGTCT 50.0 50.0 57.9 58.0 2126-2597 bp 0.875 81.5
trnT-UGU-ccsA_p2 CTCAGAGGTTAGAGCATCGC CAGCTCTTTTATGCGGGTCT 55.0 50.0 57.9 58.0 1674-2132 bp 0.875 81.4
trnT-UGU-ccsA_p3 TCGGATTCTGGATCACGAGA GCGACATCAGGTTTCTGTCT 50.0 50.0 57.9 57.9 2238-2709 bp 0.875 81.0
trnT-UGU-ccsA_p4 TTGCATTACCAGGACTGAGC CAGCTCTTTTATGCGGGTCT 50.0 50.0 57.9 58.0 2338-2809 bp 0.875 80.9
trnT-UGU-ccsA_p5 TCGGATTCTGGATCACGAGA GTACCTGCACTGCAATCTCA 50.0 50.0 57.9 57.9 2187-2658 bp 0.875 80.9