Hotspot

ndhB-trnL-CAA

IRb · 116093-116868 · 776 bp

Back to Cyperus

Pi 0.0625

MarkerSeek score 75.30

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRb
length_bp: 776
variable_sites: 193
pi: 0.0625
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	836-2507 bp	0.875	74.7
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	834-2505 bp	0.875	74.7
ndhB-trnL-CAA_p3	TGCAATTGCTCAGGATACCC	TGTTTGAACAGCGTCAGTCA	50.0	45.0	57.9	58.0	1374-2551 bp	0.625	65.0
ndhB-trnL-CAA_p4	TGCAATTGCTCAGGATACCC	TCCATAACCGCAAGTGCTAC	50.0	50.0	57.9	58.0	1421-2598 bp	0.625	64.9
ndhB-trnL-CAA_p5	TGCAATTGCTCAGGATACCC	ATAACCGCAAGTGCTACAGG	50.0	50.0	57.9	58.0	1418-2595 bp	0.625	64.9