Hotspot

rps12-psbB

LSC · 73424-75224 · 1801 bp

Back to Cyperus

Pi 0.0108

MarkerSeek score 61.10

Primer available yes

Label rps12-psbB

Feature Metadata

label_name: rps12-psbB
feature_type: igs
region: LSC
length_bp: 1801
variable_sites: 64
pi: 0.0108
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-psbB_p1	GGACATCCTTTAAGAGCGGG	CAATCGACCGGGATCATTCA	55.0	50.0	58.0	57.8	1915-2052 bp	1.0	82.2
rps12-psbB_p2	GGACATCCTTTAAGAGCGGG	TAAAGCCATTGAACCAGCCC	55.0	50.0	58.0	58.4	1975-2112 bp	1.0	80.5
rps12-psbB_p3	GGACATCCTTTAAGAGCGGG	GAACAGAAAGCAATCGACCG	55.0	50.0	58.0	57.5	1925-2062 bp	1.0	80.0
rps12-psbB_p4	GGACATCCTTTAAGAGCGGG	GCAACTAGAGCTGTGTGCAT	55.0	50.0	58.0	58.6	1950-2087 bp	1.0	79.6
rps12-psbB_p5	GGACATCCTTTAAGAGCGGG	AACAGAAAGCAATCGACCGG	55.0	50.0	58.0	58.8	1924-2061 bp	1.0	77.4