Hotspot

trnY-GUA-trnD-GUC

LSC · 32627-33184 · 558 bp

Back to Cyperus

Pi 0.0231
MarkerSeek score 70.20
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
558
variable_sites
37
pi
0.0231
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 TTAATCCTTCCACCCAGGGT TCGGATTCTAGTTCGGGACT 50.0 50.0 57.9 57.6 684-745 bp 1.0 84.1
trnY-GUA-trnD-GUC_p2 TTAATCCTTCCACCCAGGGT CGGATTCTAGTTCGGGACTG 50.0 55.0 57.9 57.5 683-744 bp 1.0 83.6
trnY-GUA-trnD-GUC_p3 TTAATCCTTCCACCCAGGGT GCGGTGCTCTAACCAATTGA 50.0 50.0 57.9 58.6 628-689 bp 1.0 83.4
trnY-GUA-trnD-GUC_p4 GGGGACGGACTGTAAATTCG TCGGATTCTAGTTCGGGACT 55.0 50.0 58.4 57.6 645-706 bp 1.0 82.0
trnY-GUA-trnD-GUC_p5 GGGACGGACTGTAAATTCGT TCGGATTCTAGTTCGGGACT 50.0 50.0 57.6 57.6 644-705 bp 1.0 81.8