Hotspot

ndhG-ndhI

SSC · 122302-122756 · 455 bp

Back to Cynometra

Pi 0.0136

MarkerSeek score 46.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 455
variable_sites: 11
pi: 0.0136
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TGTATTGGTTCAGGCAAATCCA	TTCTTTGGGCCGTTTACCAA	40.9	45.0	58.2	57.9	557-628 bp	1.0	63.1
ndhG-ndhI_p2	TGTATTGGTTCAGGCAAATCCA	TTTCTTTGGGCCGTTTACCA	40.9	45.0	58.2	57.9	558-629 bp	1.0	63.1
ndhG-ndhI_p3	TGTATTGGTTCAGGCAAATCCA	TTTGGGCCGTTTACCAATGT	40.9	45.0	58.2	58.3	554-625 bp	1.0	61.3
ndhG-ndhI_p4	ATTGGTTCAGGCAAATCCAT	TTCTTTGGGCCGTTTACCAA	40.0	45.0	55.5	57.9	554-625 bp	1.0	61.3
ndhG-ndhI_p5	ATTGGTTCAGGCAAATCCAT	TTTCTTTGGGCCGTTTACCA	40.0	45.0	55.5	57.9	555-626 bp	1.0	61.3