Hotspot

ccsA-psaC

SSC · 118773-120697 · 1925 bp

Back to Cynometra

Pi 0.00697
MarkerSeek score 48.70
Primer available yes
Label ccsA-psaC

Feature Metadata

label_name
ccsA-psaC
feature_type
igs
region
SSC
length_bp
1925
variable_sites
23
pi
0.00697
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-psaC_p1 AAGCACAGTAATCGCACCAA TCTGCTCCAAGAACAGAGGA 45.0 50.0 58.1 58.0 2713-2718 bp 1.0 80.2
ccsA-psaC_p2 AATGAGGCATGGGGATCCTA TCTGCTCCAAGAACAGAGGA 50.0 50.0 57.9 58.0 2268-2273 bp 1.0 80.1
ccsA-psaC_p3 TAATGAGGCATGGGGATCCT TCTGCTCCAAGAACAGAGGA 50.0 50.0 57.9 58.0 2269-2274 bp 1.0 80.1
ccsA-psaC_p4 TGAGGCATGGGGATCCTATT TCTGCTCCAAGAACAGAGGA 50.0 50.0 57.9 58.0 2266-2271 bp 1.0 80.1
ccsA-psaC_p5 GCTAATGAGGCATGGGGATC TCTGCTCCAAGAACAGAGGA 55.0 50.0 58.2 58.0 2271-2276 bp 1.0 79.9