Hotspot

rpl32-trnL-UAG

SSC · 116431-117648 · 1218 bp

Back to Cynometra

Pi 0.0111
MarkerSeek score 62.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1218
variable_sites
24
pi
0.0111
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCCACATCTCTTTCTACGGG AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 1313-1369 bp 1.0 82.2
rpl32-trnL-UAG_p2 GCCACATCTCTTTCTACGGG CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 1314-1370 bp 1.0 82.2
rpl32-trnL-UAG_p3 ACGGGGAACTCAAAAAGCTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 1298-1354 bp 1.0 82.2
rpl32-trnL-UAG_p4 ACGGGGAACTCAAAAAGCTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 1299-1355 bp 1.0 82.2
rpl32-trnL-UAG_p5 GCCACATCTCTTTCTACGGG TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.1 57.9 1323-1379 bp 1.0 81.3