Hotspot

rps3-rps19

LSC · 85989-86615 · 627 bp

Back to Cynometra

Pi 0.00481

MarkerSeek score 60.00

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 627
variable_sites: 5
pi: 0.00481
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	CTCTAAACTTTCGGGGGCAC	50.0	55.0	57.5	58.5	694-717 bp	1.0	75.7
rps3-rps19_p2	GTCTGAAACCGAGCGGATTT	CTCTAAACTTTCGGGGGCAC	50.0	55.0	58.6	58.5	688-711 bp	1.0	74.9
rps3-rps19_p3	TACCAAGTCTGAAACCGAGC	GGGCACGGCAAAAATGATAA	50.0	45.0	57.5	57.3	680-703 bp	1.0	74.9
rps3-rps19_p4	GTCTGAAACCGAGCGGATTT	GGGCACGGCAAAAATGATAA	50.0	45.0	58.6	57.3	674-697 bp	1.0	74.1
rps3-rps19_p5	TACCAAGTCTGAAACCGAGC	ACTCTAAACTTTCGGGGGCA	50.0	50.0	57.5	58.9	695-718 bp	1.0	72.6