Hotspot

rps8-rpl14

LSC · 82956-83164 · 209 bp

Back to Cynometra

Pi 0.00481

MarkerSeek score 61.60

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 209
variable_sites: 2
pi: 0.00481
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ATTCGAACCGTTCCCTTTCT	TCGCACGAGAATTGAGACAG	45.0	50.0	57.1	58.0	340-351 bp	1.0	76.0
rps8-rpl14_p2	ATTCGAACCGTTCCCTTTCT	GTTTCATTAGCACCCGAGGT	45.0	50.0	57.1	57.9	302-313 bp	1.0	75.7
rps8-rpl14_p3	ATTCGAACCGTTCCCTTTCT	CGATCGCACGAGAATTGAGA	45.0	50.0	57.1	58.2	343-354 bp	1.0	74.8
rps8-rpl14_p4	CAGCAATCGTATCCTTTCCCA	TCGCACGAGAATTGAGACAG	47.6	50.0	58.1	58.0	285-296 bp	1.0	74.8
rps8-rpl14_p5	ATTCGAACCGTTCCCTTTCT	AGAATTTTTGGTGCGATCGC	45.0	45.0	57.1	57.7	356-367 bp	1.0	73.9