Hotspot

trnF-GAA-ndhJ

LSC · 50640-51335 · 696 bp

Back to Cynometra

Pi 0.00339

MarkerSeek score 62.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 4
pi: 0.00339
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATGCCCGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	839-847 bp	1.0	78.3
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	803-811 bp	1.0	78.0
trnF-GAA-ndhJ_p3	AGAATGGTCGGGATAGCTCA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	57.9	57.7	848-856 bp	1.0	77.6
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	ATGCCCGAAAGTTGGATAGG	55.0	50.0	57.9	57.7	827-835 bp	1.0	77.3
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GCCCGAAAGTTGGATAGGAT	55.0	50.0	58.0	57.4	837-845 bp	1.0	76.0