Hotspot

petN-psbM

LSC · 29280-30538 · 1259 bp

Back to Cynometra

Pi 0.00743

MarkerSeek score 64.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1259
variable_sites: 18
pi: 0.00743
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1417-1421 bp	1.0	68.9
petN-psbM_p2	CCCTCGTAGTATGGGGAAGA	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.6	57.6	1366-1370 bp	1.0	67.2
petN-psbM_p3	TCCCTCGTAGTATGGGGAAG	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.6	57.6	1367-1371 bp	1.0	67.2
petN-psbM_p4	GGGGGCATAATTCACATGGA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1439-1443 bp	1.0	67.1
petN-psbM_p5	AGGGGGCATAATTCACATGG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1440-1444 bp	1.0	67.0