Hotspot

trnC-GCA-petN

LSC · 28108-29189 · 1082 bp

Back to Cynometra

Pi 0.0037

MarkerSeek score 44.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1082
variable_sites: 7
pi: 0.0037
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1138-1161 bp	1.0	79.5
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TCTTCCCCATACTACGAGGG	50.0	55.0	58.1	57.6	1189-1212 bp	1.0	77.6
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	1156-1179 bp	1.0	77.2
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	TCTTCCCCATACTACGAGGG	50.0	55.0	58.4	57.6	1207-1230 bp	1.0	75.4
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	TCCCCATACTACGAGGGAAA	50.0	50.0	58.1	57.1	1186-1209 bp	1.0	73.8