Hotspot

trnS-GCU-trnG-UCC

LSC · 7269-8124 · 856 bp

Back to Cynometra

Pi 0.0132

MarkerSeek score 60.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 856
variable_sites: 20
pi: 0.0132
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ATGAATCAAACGAGGGACCC	50.0	50.0	58.0	57.6	945-989 bp	1.0	80.8
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ATGAATCAAACGAGGGACCC	50.0	50.0	58.0	57.6	944-988 bp	1.0	80.8
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	ATGAATCAAACGAGGGACCC	50.0	50.0	58.2	57.6	999-1043 bp	1.0	79.4
trnS-GCU-trnG-UCC_p4	AGCAATCCGACGCTTTAGTC	ATGAATCAAACGAGGGACCC	50.0	50.0	58.4	57.6	947-991 bp	1.0	77.9
trnS-GCU-trnG-UCC_p5	CGACGCTTTAGTCCACTCAG	ATGAATCAAACGAGGGACCC	55.0	50.0	58.4	57.6	940-984 bp	1.0	77.8