Hotspot

trnV-GAC

IRa · 123215-123286 · 72 bp

Back to Cynodon

Pi 0.0556
MarkerSeek score 81.90
Primer available yes
Label trnV-GAC

Feature Metadata

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC_p1 CTTGAGTTTCTCGCCCCTTT ACCCTATCACGTCAATCCCA 50.0 50.0 58.1 58.1 225-235 bp 1.0 79.2
trnV-GAC_p2 CTTGAGTTTCTCGCCCCTTT GTCAATCCCACAAGCCTCTT 50.0 50.0 58.1 57.8 215-225 bp 1.0 78.6
trnV-GAC_p3 CTTGAGTTTCTCGCCCCTTT CCCTATCACGTCAATCCCAC 50.0 55.0 58.1 57.8 224-234 bp 1.0 78.3
trnV-GAC_p4 ATAGTGCGGAAAGAAAGCCC ACCCTATCACGTCAATCCCA 50.0 50.0 58.2 58.1 264-274 bp 1.0 77.9
trnV-GAC_p5 CTTGAGTTTCTCGCCCCTTT CACCAGCAGTATAGCCAACC 50.0 55.0 58.1 58.3 247-257 bp 1.0 77.5
trnV-GAC_p1 ACCCTATCACGTCAATCCCA CTTGAGTTTCTCGCCCCTTT 50.0 50.0 58.1 58.1 225-233 bp 1.0 79.6
trnV-GAC_p2 GTCAATCCCACAAGCCTCTT CTTGAGTTTCTCGCCCCTTT 50.0 50.0 57.8 58.1 215-223 bp 1.0 79.0
trnV-GAC_p3 CCCTATCACGTCAATCCCAC CTTGAGTTTCTCGCCCCTTT 55.0 50.0 57.8 58.1 224-232 bp 1.0 78.7
trnV-GAC_p4 ACCCTATCACGTCAATCCCA ATAGTGCGGAAAGAAAGCCC 50.0 50.0 58.1 58.2 264-272 bp 1.0 78.2
trnV-GAC_p5 CACCAGCAGTATAGCCAACC CTTGAGTTTCTCGCCCCTTT 55.0 50.0 58.3 58.1 247-255 bp 1.0 77.8