Hotspot

rrn16

IRa · 121494-122985 · 1492 bp

Back to Cynodon

Pi 0.00134

MarkerSeek score 59.50

Primer available yes

Label rrn16

Feature Metadata

label_name: rrn16
feature_type: rRNA
region: IRa
length_bp: 1492
variable_sites: 2
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16_p1	CAGACGTAGCTGCCTTCTTT	CCGAATCCGCTTTGTCTACA	50.0	50.0	57.9	58.0	1624-1635 bp	1.0	79.4
rrn16_p2	CAGACGTAGCTGCCTTCTTT	TCCGAATCCGCTTTGTCTAC	50.0	50.0	57.9	57.7	1625-1636 bp	1.0	77.1
rrn16_p3	CCTTCTTTTTGGGCGTGAAG	CCGAATCCGCTTTGTCTACA	50.0	50.0	57.6	58.0	1612-1623 bp	1.0	76.7
rrn16_p4	CAGACGTAGCTGCCTTCTTT	AGACAATTCCGAATCCGCTT	50.0	45.0	57.9	57.6	1632-1643 bp	1.0	76.2
rrn16_p5	CAGACGTAGCTGCCTTCTTT	ATTCCGAATCCGCTTTGTCT	50.0	45.0	57.9	57.6	1627-1638 bp	1.0	76.2