Hotspot

rpl22

LSC · 79245-79694 · 450 bp

Back to Cynodon

Pi 0

MarkerSeek score 23.70

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 450
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	ACCAAGTCTGAAACCGAGTG	GGTAGGCCGGAAATAACCTT	50.0	50.0	57.8	57.3	572 bp	1.0	72.3
rpl22_p2	CCAAGTCTGAAACCGAGTGG	GGTAGGCCGGAAATAACCTT	55.0	50.0	58.5	57.3	571 bp	1.0	70.3
rpl22_p3	CAAGTCTGAAACCGAGTGGA	GGTAGGCCGGAAATAACCTT	50.0	50.0	57.5	57.3	570 bp	1.0	70.0
rpl22_p4	CAAGTCTGAAACCGAGTGGAT	GGTAGGCCGGAAATAACCTT	47.6	50.0	58.0	57.3	570 bp	1.0	65.8
rpl22_p5	TGGTGATTTTGGGTTGTACCA	GGTAGGCCGGAAATAACCTT	42.9	50.0	57.7	57.3	589 bp	1.0	63.7