Hotspot

rpl16

LSC · 76907-78364 · 1458 bp

Back to Cynodon

Pi 0.00137

MarkerSeek score 59.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1458
variable_sites: 2
pi: 0.00137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CTCTCCAGAAGGAAAACCCG	AGACAAGAGCCTTATTTTTCCA	55.0	36.4	57.9	55.6	1627-1628 bp	1.0	44.3
rpl16_p2	CTCTCCAGAAGGAAAACCCG	AGCCTTATTTTTCCAAAAATCCCA	55.0	33.3	57.9	57.6	1620-1621 bp	1.0	43.8
rpl16_p3	CTCTCCAGAAGGAAAACCCG	AAGACAAGAGCCTTATTTTTCCA	55.0	34.8	57.9	56.4	1628-1629 bp	1.0	42.2
rpl16_p4	CTCTCCAGAAGGAAAACCCG	AGACAAGAGCCTTATTTTTCCAA	55.0	34.8	57.9	56.4	1627-1628 bp	1.0	42.2
rpl16_p5	CTCTCCAGAAGGAAAACCCG	GCCTTATTTTTCCAAAAATCCCAG	55.0	37.5	57.9	57.3	1619-1620 bp	1.0	41.4