Hotspot

psbC

LSC · 10099-11520 · 1422 bp

Back to Cynodon

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTCGTTTCCCAGGAAATCCG	TGGCTCGGCTAGATGGAATA	50.0	50.0	58.2	58.0	1607 bp	1.0	78.5
psbC_p2	GCAGCGGAAGATCCTGAATT	TGGCTCGGCTAGATGGAATA	50.0	50.0	58.3	58.0	1586 bp	1.0	77.3
psbC_p3	TTCGTTTCCCAGGAAATCCG	ATGAATGGCTCGGCTAGATG	50.0	50.0	58.2	57.5	1612 bp	1.0	74.8
psbC_p4	GCAGCGGAAGATCCTGAATT	ATGAATGGCTCGGCTAGATG	50.0	50.0	58.3	57.5	1591 bp	1.0	73.6
psbC_p5	TTCGTTTCCCAGGAAATCCG	ATGGCTCGGCTAGATGGAAT	50.0	50.0	58.2	58.6	1608 bp	1.0	73.4