Hotspot

rrn16-trnV-GAC

IRa · 122986-123214 · 229 bp

Back to Cynodon

Pi 0
MarkerSeek score 22.10
Primer available yes
Label rrn16-trnV-GAC

Feature Metadata

label_name
rrn16-trnV-GAC
feature_type
igs
region
IRa
length_bp
229
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
22.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-trnV-GAC_p1 GCAGGTTCTTACGCGTTACT TTGACGTGGTGGAAGTCATC 50.0 50.0 58.3 57.8 391 bp 1.0 76.3
rrn16-trnV-GAC_p2 GCAGGTTCTTACGCGTTACT CGTGGTGGAAGTCATCAGTT 50.0 50.0 58.3 57.8 387 bp 1.0 76.3
rrn16-trnV-GAC_p3 GCAGGTTCTTACGCGTTACT CTTGACGTGGTGGAAGTCAT 50.0 50.0 58.3 57.8 392 bp 1.0 76.3
rrn16-trnV-GAC_p4 TTCTTACGCGTTACTCACCC TTGACGTGGTGGAAGTCATC 50.0 50.0 57.6 57.8 386 bp 1.0 75.9
rrn16-trnV-GAC_p5 TTCTTACGCGTTACTCACCC CGTGGTGGAAGTCATCAGTT 50.0 50.0 57.6 57.8 382 bp 1.0 75.8