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rpl22-rps19

IRb · 79695-79772 · 78 bp

Back to Cynodon

Pi 0.0769

MarkerSeek score 84.60

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 78
variable_sites: 6
pi: 0.0769
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTAGCCCGTTCTTCTTCTT	GGTAGGTCGTAAATTGGGGG	50.0	55.0	57.6	57.7	228-243 bp	1.0	76.4
rpl22-rps19_p2	TTCTTGCGAGTTTCCGTAGC	GGTAGGTCGTAAATTGGGGG	50.0	55.0	58.6	57.7	242-257 bp	1.0	74.6
rpl22-rps19_p3	GTTTCCGTAGCCCGTTCTTC	GGTAGGTCGTAAATTGGGGG	55.0	55.0	58.9	57.7	233-248 bp	1.0	71.9
rpl22-rps19_p4	TTCCGTAGCCCGTTCTTCTT	GGTAGGTCGTAAATTGGGGG	50.0	55.0	59.0	57.7	231-246 bp	1.0	71.1
rpl22-rps19_p5	TTTCCGTAGCCCGTTCTTCT	GGTAGGTCGTAAATTGGGGG	50.0	55.0	59.0	57.7	232-247 bp	1.0	71.1