Hotspot

petA-psbJ

LSC · 60238-61098 · 861 bp

Back to Cynodon

Pi 0.00697

MarkerSeek score 62.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 861
variable_sites: 6
pi: 0.00697
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTCT	ACGGTTCGTATTCTGGATTGG	50.0	47.6	58.7	58.1	1005-1010 bp	1.0	66.2
petA-psbJ_p2	GATCCATTACGCGTCCAAGG	ACGGTTCGTATTCTGGATTGG	55.0	47.6	58.8	58.1	1016-1021 bp	1.0	65.4
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	GGTTCGTATTCTGGATTGGGT	50.0	47.6	58.7	57.7	1003-1008 bp	1.0	64.8
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	GCTGATAGGTACTGTAACCGG	50.0	52.4	58.7	57.6	1061-1066 bp	1.0	64.1
petA-psbJ_p5	CGTCCAAGGCCTTTTGTTCT	GGCTGATAGGTACTGTAACCG	50.0	52.4	58.7	57.6	1062-1067 bp	1.0	64.1