Hotspot

trnG-UCC-trnT-GGU

LSC · 13776-14747 · 972 bp

Back to Cynodon

Pi 0.00928
MarkerSeek score 61.70
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
972
variable_sites
9
pi
0.00928
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT GGTCTTTCCGTGGCATTACT 38.1 50.0 55.5 57.9 1114 bp 1.0 51.6
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT TCCGTGGCATTACTCTACCA 38.1 50.0 55.5 58.1 1108 bp 1.0 51.3
trnG-UCC-trnT-GGU_p3 AGAACGAATCACACTTTTACCAC GGTCTTTCCGTGGCATTACT 39.1 50.0 57.3 57.9 1117 bp 1.0 50.6
trnG-UCC-trnT-GGU_p4 GAACGAATCACACTTTTACCACT GGTCTTTCCGTGGCATTACT 39.1 50.0 57.3 57.9 1116 bp 1.0 50.6
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT TCCGTGGCATTACTCTACCA 39.1 50.0 57.3 58.1 1110 bp 1.0 50.3