Hotspot

trnS-GCU-psbD

LSC · 8165-9089 · 925 bp

Back to Cynodon

Pi 0

MarkerSeek score 46.00

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 925
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	ACTCTACCAAGGGCTATAGTCA	45.0	45.5	58.0	57.5	986-988 bp	1.0	59.6
trnS-GCU-psbD_p2	GAAAGTCCCAAACGATCCGA	ACTCTACCAAGGGCTATAGTCA	50.0	45.5	57.9	57.5	1059-1061 bp	1.0	59.2
trnS-GCU-psbD_p3	TCGAAAGTCCCAAACGATCC	ACTCTACCAAGGGCTATAGTCA	50.0	45.5	57.9	57.5	1061-1063 bp	1.0	59.2
trnS-GCU-psbD_p4	AAAGTCCCAAACGATCCGAG	ACTCTACCAAGGGCTATAGTCA	50.0	45.5	57.9	57.5	1058-1060 bp	1.0	59.2
trnS-GCU-psbD_p5	GCTTTAGTCCACTCAGCCAT	ACTCTACCAAGGGCTATAGTCA	50.0	45.5	57.6	57.5	972-974 bp	1.0	56.7