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matK-trnK-UUU

LSC · 3213-3883 · 671 bp

Back to Cynodon

Pi 0.00298

MarkerSeek score 60.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 2
pi: 0.00298
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CATAATCGTGGGCAAATGCA	CAGGAGCGTTCGGTGTAATT	45.0	50.0	57.1	58.3	880 bp	1.0	71.0
matK-trnK-UUU_p2	ACATAATCGTGGGCAAATGC	CAGGAGCGTTCGGTGTAATT	45.0	50.0	57.1	58.3	881 bp	1.0	71.0
matK-trnK-UUU_p3	CATAATCGTGGGCAAATGCA	AGGAGCGTTCGGTGTAATTT	45.0	45.0	57.1	57.5	879 bp	1.0	69.5
matK-trnK-UUU_p4	ACATAATCGTGGGCAAATGC	AGGAGCGTTCGGTGTAATTT	45.0	45.0	57.1	57.5	880 bp	1.0	69.5
matK-trnK-UUU_p5	CATAATCGTGGGCAAATGCAT	CAGGAGCGTTCGGTGTAATT	42.9	50.0	57.6	58.3	880 bp	1.0	67.0